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Fluorescent probing for RNA molecules by an unnatural base-pair system

Fluorescent labeling of nucleic acids is widely used in basic research and medical applications. We describe the efficient site-specific incorporation of a fluorescent base analog, 2-amino-6-(2-thienyl)purine (s), into RNA by transcription mediated by an unnatural base pair between s and pyrrole-2-c...

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Detalhes bibliográficos
Main Authors: Kimoto, Michiko, Mitsui, Tsuneo, Harada, Yoko, Sato, Akira, Yokoyama, Shigeyuki, Hirao, Ichiro
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2007
Assuntos:
RNA
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2018647/
https://ncbi.nlm.nih.gov/pubmed/17693436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm508
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