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Catalyst-Free and Site-Specific One-Pot Dual Labeling of a Protein Directed by Two Genetically Incorporated Noncanonical Amino Acids

[Image: see text] The genetic incorporation of one azide-containing and one keto-containing noncanonical amino acids into a protein at one amber and one ochre mutation sites respectively followed by their orthogonal reactions with one hydroxylamine-containing and one cyclooctyne-containing dyes allo...

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Hlavní autoři: Wu, Bo, Wang, Zhiyong, Huang, Ying, Liu, Wenshe R.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3389564/
https://ncbi.nlm.nih.gov/pubmed/22628069
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201200281
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