Automated specimen search in cryo-TEM observation with DIFF-defocus imaging

We have developed an automated specimen search algorithm for cryo-electron microscopy imaging of ice-embedded single particles suspended across regularly spaced holes. To maximize the particle visibility under a low electron exposure rate condition, specimen searching is carried out in diffraction m...

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Detalhes bibliográficos
Main Authors: Nakamura, Natsuko, Shimizu, Yuko, Shinkawa, Takao, Nakata, Munetaka, Bammes, Benjamin, Zhang, Junjie, Chiu, Wah
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2010
Assuntos:
Biological: Full-length
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388909/
https://ncbi.nlm.nih.gov/pubmed/20356853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jmicro/dfq009
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