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Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 and U5 snRNPs.

The four major nucleoplasmic small nuclear ribonucleoprotein particles U1, U2, U4/U6 and U5 can be extensively purified from HeLa cells by immunoaffinity chromatography using a monoclonal anti-trimethylguanosine antibody. The snRNP particles in active splicing extracts are selectively bound to the i...

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Bibliographic Details
Main Author: Krainer, A R
Format: Artigo
Language:Inglês
Published: 1988
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC338753/
https://ncbi.nlm.nih.gov/pubmed/3141901
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