Rapid hierarchical assembly of medium-size DNA cassettes

Synthetic biology applications call for efficient methods to generate large gene cassettes that encode complex gene circuits in order to avoid simultaneous delivery of multiple plasmids encoding individual genes. Multiple methods have been proposed to achieve this goal. Here, we describe a novel pro...

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Bibliografske podrobnosti
Main Authors: Schmid-Burgk, Jonathan Leo, Xie, Zhen, Frank, Stefan, Virreira Winter, Sebastian, Mitschka, Sibylle, Kolanus, Waldemar, Murray, Andrew, Benenson, Yaakov
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2012
Teme:
Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3384347/
https://ncbi.nlm.nih.gov/pubmed/22422837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks236
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