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Rapid hierarchical assembly of medium-size DNA cassettes

Synthetic biology applications call for efficient methods to generate large gene cassettes that encode complex gene circuits in order to avoid simultaneous delivery of multiple plasmids encoding individual genes. Multiple methods have been proposed to achieve this goal. Here, we describe a novel pro...

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Detalhes bibliográficos
Main Authors: Schmid-Burgk, Jonathan Leo, Xie, Zhen, Frank, Stefan, Virreira Winter, Sebastian, Mitschka, Sibylle, Kolanus, Waldemar, Murray, Andrew, Benenson, Yaakov
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3384347/
https://ncbi.nlm.nih.gov/pubmed/22422837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks236
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