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Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.

A lambda insertion type cDNA cloning vector, Lambda ZAP, has been constructed. In E. coli a phagemid, pBluescript SK(-), contained within the vector, can be excised by f1 or M13 helper phage. The excision process eliminates the need to subclone DNA inserts from the lambda phage into a plasmid by res...

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Main Authors: Short, J M, Fernandez, J M, Sorge, J A, Huse, W D
格式: Artigo
語言:Inglês
出版: 1988
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC338428/
https://ncbi.nlm.nih.gov/pubmed/2970625
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