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Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.

A lambda insertion type cDNA cloning vector, Lambda ZAP, has been constructed. In E. coli a phagemid, pBluescript SK(-), contained within the vector, can be excised by f1 or M13 helper phage. The excision process eliminates the need to subclone DNA inserts from the lambda phage into a plasmid by res...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Short, J M, Fernandez, J M, Sorge, J A, Huse, W D
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1988
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC338428/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2970625/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.15.7583
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