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Global identification of protein kinase substrates by protein microarray analysis
We describe a protocol for the global identification of the in vitro substrates targeted by protein kinases using protein microarray technology. Large numbers of fusion proteins TAP-tagged at their carboxy-termini are purified in 96-well format and spotted in duplicate onto amino-silane coated slide...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3382020/ https://ncbi.nlm.nih.gov/pubmed/20010933 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.194 |
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