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Global identification of protein kinase substrates by protein microarray analysis

We describe a protocol for the global identification of the in vitro substrates targeted by protein kinases using protein microarray technology. Large numbers of fusion proteins TAP-tagged at their carboxy-termini are purified in 96-well format and spotted in duplicate onto amino-silane coated slide...

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Autors principals: Mok, Janine, Im, Hogune, Snyder, Michael
Format: Artigo
Idioma:Inglês
Publicat: 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3382020/
https://ncbi.nlm.nih.gov/pubmed/20010933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.194
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