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High efficiency recombineering in lactic acid bacteria

The ability to efficiently generate targeted point mutations in the chromosome without the need for antibiotics, or other means of selection, is a powerful strategy for genome engineering. Although oligonucleotide-mediated recombineering (ssDNA recombineering) has been utilized in Escherichia coli f...

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Autori principali: van Pijkeren, Jan-Peter, Britton, Robert A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3378904/
https://ncbi.nlm.nih.gov/pubmed/22328729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks147
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