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T7 RNA Polymerase Bypass of Large Gaps on the Template Strand Reveals a Critical Role of the Nontemplate Strand in Elongation
We show that T7 RNA polymerase can efficiently transcribe DNA containing gaps from one to five bases in the template strand. Surprisingly, broken template strands missing up to 24 bases can still be transcribed, although at reduced efficiency. The resulting transcripts contain the full template sequ...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1995
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3375833/ https://ncbi.nlm.nih.gov/pubmed/7664337 |
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