Expression, purification, crystallization and preliminary X-ray analysis of carbonyl reductase S1 from Candida magnoliae

The NADPH-dependent carbonyl reductase S1 from Candida magnoliae stereoselectively catalyzes the reduction of ethyl 4-chloro-3-oxobutanoate (COBE) to ethyl (S)-4-chloro-3-hydroxybutanoate (CHBE), which is a chiral compound valuable as a building block for pharmaceuticals. Carbonyl reductase S1 was e...

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Main Authors: Suwa, Yoichi, Ohtsuka, Jun, Miyakawa, Takuya, Imai, Fabiana Lica, Okai, Masahiko, Sawano, Yoriko, Yasohara, Yoshihiko, Kataoka, Michihiko, Shimizu, Sakayu, Tanokura, Masaru
格式: Artigo
語言:Inglês
出版: International Union of Crystallography 2012
主題:
Crystallization Communications
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3374508/
https://ncbi.nlm.nih.gov/pubmed/22691783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112011645
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