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Thermodynamic principles for the engineering of pH-driven conformational switches and acid insensitive proteins

The general thermodynamic principles behind pH driven conformational transitions of biological macromolecules are well understood. What is less obvious is how they can be used to engineer pH switches in proteins. The acid unfolding of staphylococcal nuclease (SNase) was used to illustrate different...

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Autores principales: Bell-Upp, Peregrine, Robinson, Aaron C., Whitten, Steven, Wheeler, Erika L., Lin, Janine, Stites, Wesley E., García-Moreno E, Bertrand
Formato: Artigo
Lenguaje:Inglês
Publicado: 2011
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3373021/
https://ncbi.nlm.nih.gov/pubmed/21802194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpc.2011.06.016
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