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Extensive phosphorothioate substitution yields highly active and nuclease-resistant hairpin ribozymes.
The catalytic function of the hairpin ribozyme has been investigated by modification-interference analysis of both ribozyme and substrate, using ribonucleoside phosphorothioates. Thiophosphate substitutions in two ribozyme domains were examined by using a novel and highly efficient two-piece ribozym...
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| Main Authors: | , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1992
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC336929/ https://ncbi.nlm.nih.gov/pubmed/1377380 |
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