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Extensive phosphorothioate substitution yields highly active and nuclease-resistant hairpin ribozymes.
The catalytic function of the hairpin ribozyme has been investigated by modification-interference analysis of both ribozyme and substrate, using ribonucleoside phosphorothioates. Thiophosphate substitutions in two ribozyme domains were examined by using a novel and highly efficient two-piece ribozym...
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| Publicado no: | Nucleic Acids Res |
|---|---|
| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1992
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336929/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1377380/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.11.2835 |
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