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Metallation State of Human Manganese Superoxide Dismutase Expressed in Saccharomyces cerevisiae

Human manganese superoxide dismutase (Sod2p) has been expressed in yeast and the protein purified from isolated yeast mitochondria, yielding both the metallated protein and the less stable apoprotein in a single chromatographic step. At 30 °C growth temperature, more than half of the purified enzyme...

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Bibliografiske detaljer
Main Authors: Whittaker, Mei M., Whittaker, James W.
Format: Artigo
Sprog:Inglês
Udgivet: 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3369014/
https://ncbi.nlm.nih.gov/pubmed/22561997
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2012.04.016
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