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Metallation State of Human Manganese Superoxide Dismutase Expressed in Saccharomyces cerevisiae
Human manganese superoxide dismutase (Sod2p) has been expressed in yeast and the protein purified from isolated yeast mitochondria, yielding both the metallated protein and the less stable apoprotein in a single chromatographic step. At 30 °C growth temperature, more than half of the purified enzyme...
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| Main Authors: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2012
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3369014/ https://ncbi.nlm.nih.gov/pubmed/22561997 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2012.04.016 |
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