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Metallation State of Human Manganese Superoxide Dismutase Expressed in Saccharomyces cerevisiae

Human manganese superoxide dismutase (Sod2p) has been expressed in yeast and the protein purified from isolated yeast mitochondria, yielding both the metallated protein and the less stable apoprotein in a single chromatographic step. At 30 °C growth temperature, more than half of the purified enzyme...

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Detalhes bibliográficos
Main Authors: Whittaker, Mei M., Whittaker, James W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3369014/
https://ncbi.nlm.nih.gov/pubmed/22561997
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2012.04.016
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