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Kinetic Analysis of the Metal Binding Mechanism of Escherichia coli Manganese Superoxide Dismutase

The acquisition of a catalytic metal cofactor is an essential step in the maturation of every metalloenzyme, including manganese superoxide dismutase (MnSOD). In this study, we have taken advantage of the quenching of intrinsic protein fluorescence by bound metal ions to continuously monitor the met...

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Autors principals: Whittaker, Mei M., Mizuno, Kazunori, Bächinger, Hans Peter, Whittaker, James W.
Format: Artigo
Idioma:Inglês
Publicat: Biophysical Society 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1367064/
https://ncbi.nlm.nih.gov/pubmed/16258041
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.071308
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