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Kinetic Analysis of the Metal Binding Mechanism of Escherichia coli Manganese Superoxide Dismutase
The acquisition of a catalytic metal cofactor is an essential step in the maturation of every metalloenzyme, including manganese superoxide dismutase (MnSOD). In this study, we have taken advantage of the quenching of intrinsic protein fluorescence by bound metal ions to continuously monitor the met...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Biophysical Society
2006
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1367064/ https://ncbi.nlm.nih.gov/pubmed/16258041 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.071308 |
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