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Use of RNase H and primer extension to analyze RNA splicing.

A new method for the characterization of pre-mRNA splicing products is presented. In this method RNA molecules are hybridized to an oligodeoxynucleotide complementary to exon sequences upstream of a given 5' splice site, and the RNA strands of the resulting RNA:DNA hybrids are cleaved by RNase...

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Autors principals: Erster, S H, Finn, L A, Frendewey, D A, Helfman, D M
Format: Artigo
Idioma:Inglês
Publicat: 1988
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC336843/
https://ncbi.nlm.nih.gov/pubmed/2840638
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