Use of RNase H and primer extension to analyze RNA splicing.

A new method for the characterization of pre-mRNA splicing products is presented. In this method RNA molecules are hybridized to an oligodeoxynucleotide complementary to exon sequences upstream of a given 5' splice site, and the RNA strands of the resulting RNA:DNA hybrids are cleaved by RNase H. Th...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Erster, S H, Finn, L A, Frendewey, D A, Helfman, D M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1988
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336843/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2840638/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.13.5999
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