Use of RNase H and primer extension to analyze RNA splicing.
A new method for the characterization of pre-mRNA splicing products is presented. In this method RNA molecules are hybridized to an oligodeoxynucleotide complementary to exon sequences upstream of a given 5' splice site, and the RNA strands of the resulting RNA:DNA hybrids are cleaved by RNase H. Th...
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| Vydáno v: | Nucleic Acids Res |
|---|---|
| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1988
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336843/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2840638/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.13.5999 |
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