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RglB facilitated cloning of highly methylated eukaryotic DNA: the human L1 transposon, plant DNA, and DNA methylated in vitro with human DNA methyltransferase.

In vitro methylation of Bluescribe plasmid DNA (pBS) with human placental DNA methyltransferase to 6% 5-methylcytosine (mC) reduced transformation efficiencies in rglB+ host strains C600 and DS410 by almost 2 orders of magnitude. By contrast, the rglB- derivative of DS410 showed no reduction in tran...

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Udgivet i:Nucleic Acids Res
Principais autores: Woodcock, D M, Crowther, P J, Diver, W P, Graham, M, Bateman, C, Baker, D J, Smith, S S
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1988
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336642/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2837736/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.10.4465
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