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T7 DNA polymerase in automated dideoxy sequencing.

T7 DNA polymerase with chemically inactivated 3'-5' exo-nuclease activity, as well as unmodified T7 DNA polymerase, were used for sequencing by the dideoxy method in an automated system with fluorescence labelled primer and on-line detection of laser-excited reaction products. An analysis...

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Autores principales: Kristensen, T, Voss, H, Schwager, C, Stegemann, J, Sproat, B, Ansorge, W
Formato: Artigo
Lenguaje:Inglês
Publicado: 1988
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC336507/
https://ncbi.nlm.nih.gov/pubmed/3375061
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