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T7 DNA polymerase in automated dideoxy sequencing.

T7 DNA polymerase with chemically inactivated 3'-5' exo-nuclease activity, as well as unmodified T7 DNA polymerase, were used for sequencing by the dideoxy method in an automated system with fluorescence labelled primer and on-line detection of laser-excited reaction products. An analysis of signal...

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Publicat a:Nucleic Acids Res
Autors principals: Kristensen, T, Voss, H, Schwager, C, Stegemann, J, Sproat, B, Ansorge, W
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1988
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336507/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3375061/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.8.3487
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