T7 DNA polymerase in automated dideoxy sequencing.

T7 DNA polymerase with chemically inactivated 3'-5' exo-nuclease activity, as well as unmodified T7 DNA polymerase, were used for sequencing by the dideoxy method in an automated system with fluorescence labelled primer and on-line detection of laser-excited reaction products. An analysis of signal...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Kristensen, T, Voss, H, Schwager, C, Stegemann, J, Sproat, B, Ansorge, W
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1988
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336507/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3375061/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.8.3487
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