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Improved negative selection protocol for Plasmodium berghei in the rodent malarial model

An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluorocytosine...

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Detalhes bibliográficos
Main Authors: Orr, Rachael Y, Philip, Nisha, Waters, Andrew P
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3364864/
https://ncbi.nlm.nih.gov/pubmed/22463060
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2875-11-103
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