Covalent cross-linking of poly(A) to Escherichia coli ribosomes, and localization of the cross-link site within the 16S RNA.
Poly(A) can be cross-linked to E. coli 70S ribosomes in the presence of tRNALys by mild ultraviolet irradiation. The cross-linking reaction is exclusively with the 30S subunit, and involves primarily the RNA moiety. Following a partial nuclease digestion, cross-linked complexes containing poly(A) an...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1988
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| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336378/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3283702/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.6.2369 |
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