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Covalent cross-linking of poly(A) to Escherichia coli ribosomes, and localization of the cross-link site within the 16S RNA.

Poly(A) can be cross-linked to E. coli 70S ribosomes in the presence of tRNALys by mild ultraviolet irradiation. The cross-linking reaction is exclusively with the 30S subunit, and involves primarily the RNA moiety. Following a partial nuclease digestion, cross-linked complexes containing poly(A) an...

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Autors principals: Stiege, W, Stade, K, Schüler, D, Brimacombe, R
Format: Artigo
Idioma:Inglês
Publicat: 1988
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC336378/
https://ncbi.nlm.nih.gov/pubmed/3283702
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