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A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA.

A method is presented for choosing optimal oligodeoxyribonucleotides as probes for filter hybridization, primers for sequencing, or primers for DNA amplification. Three main factors that determine the quality of a probe are considered: stability of the duplex formed between the probe and target nucl...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Rychlik, W, Rhoads, R E
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1989
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC335026/
https://ncbi.nlm.nih.gov/pubmed/2587212
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