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A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA.
A method is presented for choosing optimal oligodeoxyribonucleotides as probes for filter hybridization, primers for sequencing, or primers for DNA amplification. Three main factors that determine the quality of a probe are considered: stability of the duplex formed between the probe and target nucl...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1989
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC335026/ https://ncbi.nlm.nih.gov/pubmed/2587212 |
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