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A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA.

A method is presented for choosing optimal oligodeoxyribonucleotides as probes for filter hybridization, primers for sequencing, or primers for DNA amplification. Three main factors that determine the quality of a probe are considered: stability of the duplex formed between the probe and target nucl...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Rychlik, W, Rhoads, R E
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 1989
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335026/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2587212/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.21.8543
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