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Optimization of the annealing temperature for DNA amplification in vitro.

In the polymerase chain reaction (PCR) technique, DNA is amplified in vitro by a series of polymerization cycles consisting of three temperature-dependent steps: DNA denaturation, primer-template annealing, and DNA synthesis by a thermostable DNA polymerase. The purity and yield of the reaction prod...

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Detalles Bibliográficos
Main Authors: Rychlik, W, Spencer, W J, Rhoads, R E
Formato: Artigo
Idioma:Inglês
Publicado: 1990
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC332522/
https://ncbi.nlm.nih.gov/pubmed/2243783
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