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Optimization of the annealing temperature for DNA amplification in vitro.
In the polymerase chain reaction (PCR) technique, DNA is amplified in vitro by a series of polymerization cycles consisting of three temperature-dependent steps: DNA denaturation, primer-template annealing, and DNA synthesis by a thermostable DNA polymerase. The purity and yield of the reaction prod...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1990
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC332522/ https://ncbi.nlm.nih.gov/pubmed/2243783 |
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