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RNA-protein cross-linking in Escherichia coli 50S ribosomal subunits; determination of sites on 23S RNA that are cross-linked to proteins L2, L4, L24 and L27 by treatment with 2-iminothiolane.

RNA-protein cross-links were introduced into E. coli 50S ribosomal subunits by treatment with 2-iminothiolane followed by mild ultraviolet irradiation. After partial digestion of the RNA, the cross-linked RNA-protein complexes were separated by our recently published three-step procedure. In cases w...

詳細記述

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書誌詳細
出版年:Nucleic Acids Res
主要な著者: Gulle, H, Hoppe, E, Osswald, M, Greuer, B, Brimacombe, R, Stöffler, G
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 1988
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334721/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3278299/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.3.815
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