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RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S7, S9, S10, S11, S17, S18 and S21 by treatment with bis-(2-chloroethyl)-methylamine.

RNA-protein cross-links were introduced into E. coli 30S ribosomal subunits by treatment with bis-(2-chloroethyl)-methylamine. After partial nuclease digestion of the RNA moiety, a number of cross-linked RNA-protein complexes were isolated by a new three-step procedure. Protein and RNA analysis of t...

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Bibliographic Details
Main Authors: Greuer, B, Osswald, M, Brimacombe, R, Stöffler, G
Format: Artigo
Language:Inglês
Published: 1987
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC340727/
https://ncbi.nlm.nih.gov/pubmed/2437528
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