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RNA-protein cross-linking in Escherichia coli 50S ribosomal subunits; determination of sites on 23S RNA that are cross-linked to proteins L2, L4, L24 and L27 by treatment with 2-iminothiolane.

RNA-protein cross-links were introduced into E. coli 50S ribosomal subunits by treatment with 2-iminothiolane followed by mild ultraviolet irradiation. After partial digestion of the RNA, the cross-linked RNA-protein complexes were separated by our recently published three-step procedure. In cases w...

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Bibliographic Details
Main Authors: Gulle, H, Hoppe, E, Osswald, M, Greuer, B, Brimacombe, R, Stöffler, G
Format: Artigo
Language:Inglês
Published: 1988
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC334721/
https://ncbi.nlm.nih.gov/pubmed/3278299
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