RNA-protein cross-linking in Escherichia coli 50S ribosomal subunits; determination of sites on 23S RNA that are cross-linked to proteins L2, L4, L24 and L27 by treatment with 2-iminothiolane.
RNA-protein cross-links were introduced into E. coli 50S ribosomal subunits by treatment with 2-iminothiolane followed by mild ultraviolet irradiation. After partial digestion of the RNA, the cross-linked RNA-protein complexes were separated by our recently published three-step procedure. In cases w...
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| 發表在: | Nucleic Acids Res |
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| Principais autores: | , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
1988
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| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334721/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3278299/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.3.815 |
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