Strand specific cleavage of phosphorothioate-containing DNA by reaction with restriction endonucleases in the presence of ethidium bromide.
A method for achieving strand specific nicking of DNA has been developed. Phosphorothioate groups were incorporated enzymatically into the (-)strand of M13 RF IV DNA. When such DNA is reacted with restriction endonucleases in the presence of ethidium bromide nicked DNA (RF II) is produced. All of th...
-д хадгалсан:
| -д хэвлэсэн: | Nucleic Acids Res |
|---|---|
| Үндсэн зохиолчид: | , , , |
| Формат: | Artigo |
| Хэл сонгох: | Inglês |
| Хэвлэсэн: |
Oxford University Press
1988
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| Онлайн хандалт: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334720/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2830594/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.3.803 |
| Шошгууд: |
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