Strand specific cleavage of phosphorothioate-containing DNA by reaction with restriction endonucleases in the presence of ethidium bromide.
A method for achieving strand specific nicking of DNA has been developed. Phosphorothioate groups were incorporated enzymatically into the (-)strand of M13 RF IV DNA. When such DNA is reacted with restriction endonucleases in the presence of ethidium bromide nicked DNA (RF II) is produced. All of th...
Na minha lista:
| 發表在: | Nucleic Acids Res |
|---|---|
| Principais autores: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
1988
|
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334720/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2830594/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.3.803 |
| 標簽: |
沒有標簽, 成為第一個標記此記錄!
|