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Strand specific cleavage of phosphorothioate-containing DNA by reaction with restriction endonucleases in the presence of ethidium bromide.

A method for achieving strand specific nicking of DNA has been developed. Phosphorothioate groups were incorporated enzymatically into the (-)strand of M13 RF IV DNA. When such DNA is reacted with restriction endonucleases in the presence of ethidium bromide nicked DNA (RF II) is produced. All of th...

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Udgivet i:Nucleic Acids Res
Principais autores: Sayers, J R, Schmidt, W, Wendler, A, Eckstein, F
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1988
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334720/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2830594/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.3.803
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