Efficient large-scale sequencing of the Escherichia coli genome: implementation of a transposon- and PCR-based strategy for the analysis of ordered lambda phage clones.

We have developed a strategy for efficient sequence analysis of the genome of E. coli K-12 using insertions of a Tn5-derived mini-transposon into overlapping ordered lambda phage clones to provide universal primer-binding sites, and PCR amplification of DNA segments adjacent to the insertions. Trans...

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Detalhes bibliográficos
Main Authors: Kasai, H, Isono, S, Kitakawa, M, Mineno, J, Akiyama, H, Kurnit, D M, Berg, D E, Isono, K
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC334565/
https://ncbi.nlm.nih.gov/pubmed/1336178
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