Efficient large-scale sequencing of the Escherichia coli genome: implementation of a transposon- and PCR-based strategy for the analysis of ordered lambda phage clones.

We have developed a strategy for efficient sequence analysis of the genome of E. coli K-12 using insertions of a Tn5-derived mini-transposon into overlapping ordered lambda phage clones to provide universal primer-binding sites, and PCR amplification of DNA segments adjacent to the insertions. Trans...

Descrizione completa

Dettagli Bibliografici

Pubblicato in:	Nucleic Acids Res
Autori principali:	Kasai, H, Isono, S, Kitakawa, M, Mineno, J, Akiyama, H, Kurnit, D M, Berg, D E, Isono, K
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1992
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334565/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1336178/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.24.6509
Tags:	Aggiungi Tag Nessun Tag, puoi essere il primo ad aggiungerne!!