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Cloning and characterization of genes for the PvuI restriction and modification system.
The genes encoding the endonuclease and the methylase of the PvuI restriction and modification system were cloned in E.coli and characterized. The genes were adjacent in tandem orientation spanning a distance of 2200 bases. The PvuI endonuclease was a single polypeptide with a calculated molecular w...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1992
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334411/ https://ncbi.nlm.nih.gov/pubmed/1454536 |
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