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Cloning and characterization of the genes encoding the MspI restriction modification system.
The genes encoding the MspI restriction modification system, which recognizes the sequence 5' CCGG, have been cloned into pUC9. Selection was based on expression of the cloned methylase gene which renders plasmid DNA insensitive to MspI cleavage in vitro. Initially, an insert of 15 kb was obtai...
保存先:
| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1989
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317708/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2471145/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.8.3001 |
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