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Supramolecular Displacement-Mediated Activation of a Silent Fluorescence Probe for Label-free Ligand Screening

We report a new approach for the rapid screening of analyte binding affinities for a target protein. We demonstrate that a molecular probe, with a pro-fluorophore substrate and ligand moieties, can be hindered from enzymatic access when bound to the target protein. When analytes displace the probe f...

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Bibliografische gegevens
Hoofdauteurs: Torres, Diego Amado, Azagarsamy, Malar A., Thayumanavan, S.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2012
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3342419/
https://ncbi.nlm.nih.gov/pubmed/22500607
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja301204z
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