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Supramolecular Displacement-Mediated Activation of a Silent Fluorescence Probe for Label-free Ligand Screening
We report a new approach for the rapid screening of analyte binding affinities for a target protein. We demonstrate that a molecular probe, with a pro-fluorophore substrate and ligand moieties, can be hindered from enzymatic access when bound to the target protein. When analytes displace the probe f...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2012
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3342419/ https://ncbi.nlm.nih.gov/pubmed/22500607 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja301204z |
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