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Arg-302 facilitates deprotonation of Glu-325 in the transport mechanism of the lactose permease from Escherichia coli

A mechanistic model for lactose/H(+) symport via the lactose permease of Escherichia coli proposed recently indicates that the permease must be protonated to bind ligand with high affinity. Moreover, in the ground state, the symported H(+) is shared between His-322 (helix X) and Glu-269 (helix VIII)...

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Hlavní autoři: Sahin-Tóth, Miklós, Kaback, H. Ronald
Médium: Artigo
Jazyk:Inglês
Vydáno: The National Academy of Sciences 2001
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC33423/
https://ncbi.nlm.nih.gov/pubmed/11353849
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.111139698
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