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RNA ligase RtcB splices 3′-phosphate and 5′-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3′)pp(5′)G intermediates

A cherished tenet of nucleic acid enzymology holds that synthesis of polynucleotide 3′-5′ phosphodiesters proceeds via the attack of a 3′-OH on a high-energy 5′ phosphoanhydride: either a nucleoside 5′-triphosphate in the case of RNA/DNA polymerases or an adenylylated intermediate A(5′)pp(5′)N– in t...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Chakravarty, Anupam K., Subbotin, Roman, Chait, Brian T., Shuman, Stewart
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3341019/
https://ncbi.nlm.nih.gov/pubmed/22474365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1201207109
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