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Targeting of the creatine kinase M gene in embryonic stem cells using isogenic and nonisogenic vectors.
Replacement vectors with genomic DNA originating from different mouse strains were used to introduce site-specific mutations into the creatine kinase M (CKM) gene of mouse embryonic stem (ES) cells. Here we demonstrate that in mouse strain 129-derived ES cells, the gene is at least 25-fold more effi...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1992
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334053/ https://ncbi.nlm.nih.gov/pubmed/1508665 |
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