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Rapid shotgun cloning utilizing the two base recognition endonuclease CviJI.
A new approach has been developed for the rapid fragmentation and fractionation of DNA into a size suitable for shotgun cloning and sequencing. The restriction endonuclease CviJI normally cleaves the recognition sequence PuGCPy between the G and C to leave blunt ends. Atypical reaction conditions wh...
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| Główni autorzy: | , , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
1992
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| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334028/ https://ncbi.nlm.nih.gov/pubmed/1322530 |
| Etykiety: |
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