Rapid shotgun cloning utilizing the two base recognition endonuclease CviJI.

A new approach has been developed for the rapid fragmentation and fractionation of DNA into a size suitable for shotgun cloning and sequencing. The restriction endonuclease CviJI normally cleaves the recognition sequence PuGCPy between the G and C to leave blunt ends. Atypical reaction conditions wh...

Celý popis

Uloženo v:
Podrobná bibliografie
Hlavní autoři: Fitzgerald, M C, Skowron, P, Van Etten, J L, Smith, L M, Mead, D A
Médium: Artigo
Jazyk:Inglês
Vydáno: 1992
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC334028/
https://ncbi.nlm.nih.gov/pubmed/1322530
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky