Rapid shotgun cloning utilizing the two base recognition endonuclease CviJI.
A new approach has been developed for the rapid fragmentation and fractionation of DNA into a size suitable for shotgun cloning and sequencing. The restriction endonuclease CviJI normally cleaves the recognition sequence PuGCPy between the G and C to leave blunt ends. Atypical reaction conditions wh...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1992
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334028/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1322530/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.14.3753 |
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