Rapid shotgun cloning utilizing the two base recognition endonuclease CviJI.

A new approach has been developed for the rapid fragmentation and fractionation of DNA into a size suitable for shotgun cloning and sequencing. The restriction endonuclease CviJI normally cleaves the recognition sequence PuGCPy between the G and C to leave blunt ends. Atypical reaction conditions wh...

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Main Authors: Fitzgerald, M C, Skowron, P, Van Etten, J L, Smith, L M, Mead, D A
Format: Artigo
Jezik:Inglês
Izdano: 1992
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC334028/
https://ncbi.nlm.nih.gov/pubmed/1322530
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