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SLiCE: a novel bacterial cell extract-based DNA cloning method
We describe a novel cloning method termed SLiCE (Seamless Ligation Cloning Extract) that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into recombinant DNA molecules in a single in vitro recombination reaction. SLiCE overcomes the sequence limitations of tradit...
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
2012
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| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3333860/ https://ncbi.nlm.nih.gov/pubmed/22241772 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1288 |
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