High-throughput RNA interference screening using pooled shRNA libraries and next generation sequencing

RNA interference (RNAi) screening is a state-of-the-art technology that enables the dissection of biological processes and disease-related phenotypes. The commercial availability of genome-wide, short hairpin RNA (shRNA) libraries has fueled interest in this area but the generation and analysis of t...

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Hlavní autoři: Sims, David, Mendes-Pereira, Ana M, Frankum, Jessica, Burgess, Darren, Cerone, Maria-Antonietta, Lombardelli, Cristina, Mitsopoulos, Costas, Hakas, Jarle, Murugaesu, Nirupa, Isacke, Clare M, Fenwick, Kerry, Assiotis, Ioannis, Kozarewa, Iwanka, Zvelebil, Marketa, Ashworth, Alan, Lord, Christopher J
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2011
Témata:
Method
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3333774/
https://ncbi.nlm.nih.gov/pubmed/22018332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2011-12-10-r104
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